Breeding Doubled Haploid Maize Inbred Lines for Methionine and Lysine Amino Acid Composition


Maize protein quality is deficient due to lower lysine and methionine essential amino acid content. Therefore, developing
high methionine and lysine hybrids is of importance in maize. In this study, 64 maize source populations
were crossed to RWS x RWK-76 haploid inducer genotype in Sakarya, Turkey to produce haploids in 2016 and
2017. Putative haploids were planted in a greenhouse in 2017-2018 winter season in Antalya, Turkey to produce
D0 lines. 115 D1 lines were selected and planted in 2018 summer field season in Sakarya for further investigation. A
total of 65 D1 doubled haploid (DH) inbred lines were developed after final evaluations. While the majority of the
developed lines gave higher values for methionine and lysine content, Ant-QPMDH-39 and Ant-QPMDH-42 lines
were observed to have higher amino acid content when compared to parental lines. Statistics related to haploid
induction rate, germination rate, misclassification rate and doubled haploid line rate revealed that effect of source
populations cannot be ignored in DH line development. Our results showed that DH technology is efficient in line
development in terms of special traits in maize in a short breeding time.


Maize, in vivo maternal haploid, inducer, source population, amino acid quality

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