Articles

Molecular and morphometric identification of plant parasitic nematodes


Abstract


During a nematode survey in vineyards of the Veneto region (Italy), several soil samples were collected. On morphological and morphometric studies, a population from Conegliano Veneto was preliminarily identified as X. rivesi Dalmasso 1969. To support the morphological diagnosis, a molecular characterization of X. rivesi from Italy using the D2-D3 expansion segments of the 28S rDNA and the ITS containing region was undertaken. PCR-RFLP analyses were also performed to determine species-specific patterns for the Italian population of the nematode. Neighbor-Joining, Minimum Evolution and Maximum Parsimony analyses were used to reconstruct phylogenetic relationships within X. rivesi and other Xiphinema species.

Keywords

D2-D3 expansion domains; Internal Transcribed Spacers; phylogeny; Xiphinema americanum-group

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