Morphological and molecular characterization of a Steinernema carpocapsae (Nematoda Steinernematidae) strain isolated in Veneto region (Italy)

Authors

  • Giulia Torrini Consiglio per la ricerca e la sperimentazione in agricoltura – Research Centre for Agrobiology and Pedology, via Lanciola 12/A, 50125 Firenze
  • Silvia Landi Consiglio per la ricerca e la sperimentazione in agricoltura – Research Centre for Agrobiology and Pedology, via Lanciola 12/A, 50125 Firenze
  • Claudia Benvenuti Consiglio per la ricerca e la sperimentazione in agricoltura – Research Centre for Agrobiology and Pedology, via Lanciola 12/A, 50125 Firenze
  • Francesca De Luca Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Via Amendola 122/D, 70126 Bari
  • Elena Fanelli Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Via Amendola 122/D, 70126 Bari
  • Alberto Troccoli Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Via Amendola 122/D, 70126 Bari
  • Eustachio Tarasco DISSPA, Facoltà di Agraria, Università degli Studi di Bari “Aldo Moro”, Via Amendola 165/A, 70126 Bari
  • Paolo Bazzoffi Consiglio per la ricerca e la sperimentazione in agricoltura – Research Centre for Agrobiology and Pedology, via Lanciola 12/A, 50125 Firenze

Keywords:

entomopathogenic nematode, ITS region, PCR-RFLP, infectivity assay, biological control

Abstract

Veneto region (North-East Italy). This new strain was named ItS-CAO1. Molecular and morphological analyses were performed. The ITS region and the 18S rRNA gene were amplified and sequenced. The ITS products were then digested with six restriction enzymes in order to unequivocally identify this species. Nematode virulence was tested against last instar of Galleria mellonella (L.) using different laboratory assays. Insect mortality of this new strain is very high in penetration (100%) and sand column assay (93.3%) and the percentage of penetrating infective juveniles was 57.6 and 42.9, respectively. Larval mortality in one-on-one quality assay was 50% and in exposure time assay it was 50% at 19 minutes. With the results of infectivity assays we can evaluate the possibility to use this new strain in biological control programs.

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Issue

Vol. 97 (2014)

Section

Articles

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