Virus-Induced Gene Silencing in Diverse Maize Lines Using the Brome Mosaic Virus-based silencing vector

Authors

  • Larissa M Benavente US Department of Agriculture, Plant Science Research Unit, Raleigh, NC
  • Xin Shun Ding The Samuel Roberts Noble Foundation, Inc. Ardmore, OK
  • Margaret G Redinbaugh US Department of Agriculture, Corn, Soybean and Wheat Quality Research Unit, and Department of Plant Pathology, Ohio State University, Wooster, OH
  • Richard Nelson The Samuel Roberts Noble Foundation, Inc. Ardmore, OK
  • Peter Balint-Kurti US Department of Agriculture, Plant Science Research Unit, Raleigh, NC Department of Plant Pathology, North Carolina State University, Raleigh NC

Keywords:

RNA silencing, VIGS, Brome Mosaic Virus, vascular puncture, gene expression, phytoene desaturase

Abstract

Virus-induced gene silencing (VIGS) is a widely used tool for gene function studies in many plant species, though its use in cereals has been limited. In addition, within cereal species the varieties that best respond during VIGS screens are often not known. Using a Brome mosaic virus (BMV) vector designed to silence the maize phytoene desaturase (PDS) gene, a genetically diverse set of maize inbred lines was screened for development of gene silencing after inoculation of seeds through the novel use of a vascular puncture inoculation technique. In addition to Va35, which previously was shown to support silencing, maize lines NC300, Ki11, Oh7b, M162W and CML52 displayed significant visible photobleaching when challenged with the BMV-PDS. In these plants, targeted PDS mRNA expression was decreased 50-80% relative to levels in plants that were inoculated with BMV containing a fragment of the GUS gene or were mock-inoculated.

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Published

2012-12-07

Issue

Vol. 57 No. 3 (2012)

Section

Articles

License

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