Articles

Virus-Induced Gene Silencing in Diverse Maize Lines Using the Brome Mosaic Virus-based silencing vector


Abstract


Virus-induced gene silencing (VIGS) is a widely used tool for gene function studies in many plant species, though its use in cereals has been limited. In addition, within cereal species the varieties that best respond during VIGS screens are often not known. Using a Brome mosaic virus (BMV) vector designed to silence the maize phytoene desaturase (PDS) gene, a genetically diverse set of maize inbred lines was screened for development of gene silencing after inoculation of seeds through the novel use of a vascular puncture inoculation technique. In addition to Va35, which previously was shown to support silencing, maize lines NC300, Ki11, Oh7b, M162W and CML52 displayed significant visible photobleaching when challenged with the BMV-PDS. In these plants, targeted PDS mRNA expression was decreased 50-80% relative to levels in plants that were inoculated with BMV containing a fragment of the GUS gene or were mock-inoculated.

Keywords

RNA silencing; VIGS; Brome Mosaic Virus; vascular puncture; gene expression; phytoene desaturase

Full Text:

PDF





Maydica - A journal devoted to maize and allied species

ISSN: 2279-8013