Articles

Artificial inoculation of maize seeds with Sporisorium reilianum f. sp. zeae


Abstract


The objective of this research was to identify an inoculation method of Sporisorium reilianum f. sp. zeae, causal agent of the head smut of maize, which allows a high and consistent percentage of infection, for reliable selection of germplasm with genetic resistance to this pathogen. Seeds of the hybrid AZ 41801 were inoculated with telio-spores impregnated using as adherents: 1% (w/v) sodium carboxymethylcellulose (NaCMC) 1%, ADH®, 2% (w/v) sodium alginate 2%, 50% (w/v) brown sugar 50% (w/v), 2% (w/v) agar – agar, 10% (w/v) grenetin and a treatment 1: 200 (v/v) of inoculum: soil 1: 200 (v/v). Treated seeds were planted in a greenhouse in 40 x 40 cm polyethylene pots with sterile soil. In 2015, grenetin as adherent, induced 96.3% of diseased plants in greenhouse. Morphological and molecular characterization of the pathogen was carried out before the inoculation and after the finalevaluation. This method of inoculation was evaluated in 2016 in fieldconditions in Mixquiahuala, Hgo. A total of 160 lines C2-S3 from a bmaize population improvement program were inoculated with teliospores of S. reilianum f. sp. zeae using 10% grenetin as adherent. The incidence of the disease in both experiments planted in 2015 and 2016 was evalua-ted 45 days after flowering,with direct observation of symptoms and signs in male and female infloescences. One line showed 54.5% of infection. The hybrid AZ 41801 reached 70.7% of diseased plants. It is suggested to use 10% grenetin as adherent in the inoculation of maize seeds with teliospores of S. reilianum f. sp. zeae for the selection of disease resistant germplasm.

Keywords

Zea mays, head smut, artificial inoculaion with teliospores, grenetin, genetic resistance

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Maydica - A journal devoted to maize and allied species

ISSN: 2279-8013