Articles

Comparison between transgenic maize with exotic betaine aldehyde dehydrogenase (BADH) gene and its untransformed counterpart


Abstract


We investigated the performance of a transgenic maize (Zea mays L) line with an exotic betaine aldehyde dehydrogenase (BADH) gene and its untransformed counterpart under drought and normal water conditions. Membrane permeability, osmoprotectant contents, and antioxidant enzyme activities of the maize lines as well as plant height and biomass were compared. The results showed that, under drought stress, compared with the untransgenic line, the contents of glycine betaine (GB), soluble sugars, soluble proteins and proline of the trans- genic line were significantly higher, so was the peroxidase (POD) activity; the contents of superoxide anion free radical, malondialdehyde (MDA) and the electrical conductivity of the transgenic line were lower; plant height and the biomass of the transgenic line were significantly higher. Under normal water conditions, the contents of soluble protein and MDA content of the transgenic line were significantly lower; but it was not the case for the content of superoxide anion free radical, electrical conductivity and superoxide dismutase (SOD) activity. No significant difference was observed in GB content and, the plant height and the biomass between the 2 lines. We conclude that the transgenic maize with exotic BADH gene was superior over its untransformed counterpart under drought stress and they performed similarly under normal water conditions.

Keywords

BADH gene, maize, physiology, growth

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Maydica - A journal devoted to maize and allied species

ISSN: 2279-8013