Articles

Identification of QTL and candidate genes for Pb accumulation in maize at maturity stage


Abstract


Pb stress.which were located on chromosome 2 and 6 respectively. The two genes were dramatically up-regulated during led to the identification of two Pb-responsive genes from those QTLs, GRMZM2G137161 and GRMZM2G132995, in different tissues, including roots, stems, leaves, and kernels. Furthermore, RNA-seq data and qRT-PCR analysis correlated with stems. A total of 20 QTLs at logarithm of odds (LOD) ratio ≥3 were detected for Pb concentration tissues of maize were roots > leaves > stems > kernels. The Pb concentration of kernels was higher significantly stems and leaves ( P < 0.01). The present study has demonstrated that the trend of Pb concentration in different tissues and all the DH lines (P < 0.01). Pb concentration of kernels displayed significant positive relationships with leaves, and kernels were measured. There was a wide distribution of Pb concentration among the mentioned four ). Pb concentrations in roots, stems, -1 solution (500 mg kg2)3filled up with soil which was stressed with a Pb(NOidentified using 207 IBM10 DH at maturity stage. The IBM10 DH and parents were planted in pots which were important insights for protecting the environment. Quantitative trait loci (QTL) for Pb accumulation in maize were L) may provide Zea maysthe genetic basis for the underlying mechanism of tolerance for lead responses in maize (Lead (Pb) has become one of the most abundant heavy metal pollutants of the environment. Understanding

Keywords

maize, Pb concentration, quantitative trait loci (QTL), candidate gene

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Maydica - A journal devoted to maize and allied species

ISSN: 2279-8013